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Beginning of Paper
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Fluorescence Study of the
Kinetics of Energy
Coumarin 1 and Sodium fluorescein are two dyes which absorb and emit light in the visible region. By using a spectrophotometer we are recording an absorption spectrum and then determining the molar decadic absorption coefficients, which will be used later to interpret and analyse the fluo ....
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Middle of Paper
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.... obtained a spectrum with a maximum at 376.5nm at an absorbance of 1.4573, using Beer-Lambert's law, we deduced the molar decadic absorption coefficients.
Sodium fluorescein
C = 2.425 x10-5 mol dm -3
Here max was 500.5 nm at an absorbance of 2.0922
Summary of the results obtained.
Dye
max (nm)
max (m2 mol-1)
(m2 mol-1)
' (m2 mol-1)
D (Coumarin)
376.5
1.82 x 109
---
1.25 x 106
A (Fluorescein)
500.5
5.17 x 107
1.41 x 106
8 x 106
2.2. Fluorimetry
a) P ....
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SUBJECTS
